Re: [DIYbio] UV transilluminator alternative?

gelred is optimized to be a drop-in replacement for ethidium bromide,
so its excitation optima is around the deep UV border, UV-B area,
while UV includes wavelengths to 400nm the excitation efficiency for
gelred isn't so great up there. As you can see from the graph, there
is also low excitation in 500nm area.

gelgreen on the other hand is optimized to excite with more blue
light, so no need for deeper UV light sources, less worry of DNA
damage if you want to extract the gel bands

http://www.biotium.com/product/product_info/flyer/GelRed%20&%20GelGreen%20Flyer.pdf

On Mon, Jul 30, 2012 at 2:38 AM, Meow-Ludo <stuart.mckellar@gmail.com> wrote:
> Any reason for gel green over gelred? Different wavelengths of fluorescence?
>
> That photo came out alright. I can see all the bands.
>
> I have some UV led's but I am not sure what wavelength light they emit. I
> should test them at uni and see if I can see bands in the lab.
>
>
> On Monday, July 30, 2012 2:03:21 PM UTC+10, Avery wrote:
>>
>> I would recommend GelGreen and some blue LEDS.
>>
>> photo here: http://bosslab.org/
>>
>> --A
>>
>> On Sun, Jul 29, 2012 at 10:24 PM, Meow-Ludo <stuart.mckellar@gmail.com>
>> wrote:
>>>
>>> I don't have a UV transilluminator and was wondering if anyone has had
>>> any successful MacGuyver solutions?
>>>
>>> I am using GelRed and need something that will allow it to fluoresce. It
>>> does this at 312nm I believe.
>>>
>>> I have looked at the pearl biotech and iorodeo ones but they are a bit
>>> pricey at this point in time.
>>>
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>>
>>
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--
Nathan McCorkle
Rochester Institute of Technology
College of Science, Biotechnology/Bioinformatics

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