If you know any openCV, you could make an automated cell counter.
Click an image with your smartphone or microscope-mounted camera of
either a petri dish filled with colonies, or actual cells under the
microscope. Run hough circles or some other blob detection (maybe even
just a simple brightness threshold), allow the tweakable arguments to
be linked to a slider bar so if the algorithm is missing some blobs
you can visually correct it by adjusting the slider. Display a count
of the blobs.
The next version of this would be to add color discrimination, as some
cell viability assays simply add a dye to cells, and the living ones
take up the color but the dead ones don't. So then you can get a dead
to living ratio, or dead to total, living to total.
On Thu, Oct 25, 2012 at 12:51 AM, Susan Tan
<onceuponatimeforever@gmail.com> wrote:
> I'm an electrical engineer switching into software engineering; both fields
> are very similar when it comes to their reliance on programming skills. I've
> been a long-time lurker on the forums; this is my very first post. I
> currently am looking for a webapp idea for a 5-week final class project. I'm
> interested in building a webapp to help computational biologists or a
> web-app related to the field of CS-Biology. I'm not a biologist, but I do
> have the CS skills. I am not sure where to begin or how to begin. Does
> anyone have ideas to pitch to me or have resources where I can start getting
> ideas for a biology-related web application? Preferably, something not too
> ambitious, since the project should be done in 5 weeks (40hrs/week time
> commitment). Although very helpful, I don't think I am required to have a
> doctoral degree in molecular or synthetic biology in order make a
> CS-Biology-related webapp.
>
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-Nathan
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Re: [DIYbio] I am a programmer looking for webapp idea that can be done in 5 weeks!
12:32 PM |
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