Hmm if anyone would be interested in something like that in a windows app I could whip it up quickly. Ive been on a robotics team for a while and we used openCV, emguCV wrapped, extensively so this would be cake. Not trying to steal any thunder...just offering a quick solution to a somewhat trivial problem in terms of CS know-how. So cell counter with all the thresholds and tweaks broken out onto slidebars?
Sebastian S Cocioba
CEO & Founder
New York Botanics, LLC
Sent via Mobile E-Mail
On Oct 29, 2012, at 3:32 PM, Nathan McCorkle <nmz787@gmail.com> wrote:
> If you know any openCV, you could make an automated cell counter.
> Click an image with your smartphone or microscope-mounted camera of
> either a petri dish filled with colonies, or actual cells under the
> microscope. Run hough circles or some other blob detection (maybe even
> just a simple brightness threshold), allow the tweakable arguments to
> be linked to a slider bar so if the algorithm is missing some blobs
> you can visually correct it by adjusting the slider. Display a count
> of the blobs.
>
> The next version of this would be to add color discrimination, as some
> cell viability assays simply add a dye to cells, and the living ones
> take up the color but the dead ones don't. So then you can get a dead
> to living ratio, or dead to total, living to total.
>
> On Thu, Oct 25, 2012 at 12:51 AM, Susan Tan
> <onceuponatimeforever@gmail.com> wrote:
>> I'm an electrical engineer switching into software engineering; both fields
>> are very similar when it comes to their reliance on programming skills. I've
>> been a long-time lurker on the forums; this is my very first post. I
>> currently am looking for a webapp idea for a 5-week final class project. I'm
>> interested in building a webapp to help computational biologists or a
>> web-app related to the field of CS-Biology. I'm not a biologist, but I do
>> have the CS skills. I am not sure where to begin or how to begin. Does
>> anyone have ideas to pitch to me or have resources where I can start getting
>> ideas for a biology-related web application? Preferably, something not too
>> ambitious, since the project should be done in 5 weeks (40hrs/week time
>> commitment). Although very helpful, I don't think I am required to have a
>> doctoral degree in molecular or synthetic biology in order make a
>> CS-Biology-related webapp.
>>
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>
>
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> -Nathan
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Re: [DIYbio] I am a programmer looking for webapp idea that can be done in 5 weeks!
12:45 PM |
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