Re: [DIYbio] Re: Now, about that gold gun...

Here's some nice videos on Jove, on preparation of gene "bullets", and use of gene gun technology to transfect neuronal and plant tissues. Highly recommended:

http://www.jove.com/video/675/preparation-gene-gun-bullets-biolistic-transfection-neurons-slice

http://www.jove.com/video/1963/bimolecular-fluorescence-complementation-bifc-assay-for-protein

Making the gene "bullets" yourself looks entirely feasible, given the right cobbled-together equipment...

Patrik

On Monday, October 29, 2012 1:07:26 PM UTC-7, Mega wrote:

Sounds quite doable, because we have a compressor at home.


But where can you place the coated particles? I'd close the 'tube' with Parchment paper on the downside. On the top there is the compressor attached, with a electrical valve to quickly release the pressure.

Will the parchment paper allow the tungsten particles to get through, without leaving the DNA on the paper?

On Mon, Oct 29, 2012 at 6:25 PM, Cathal Garvey <cathal...@gmail.com> wrote:

Helium is used because it can achieve higher speeds, but you don't need
that much power to punch through a bit of peptidoglycan/cellulose and a
lipid bilayer. :)

On 28/10/12 19:21, Conner Berthold wrote:
> I was also looking to see if Helium could be replaced with something more
> "portable" (like CO2 cartridges) and found this article.
>
> "With the self-design CO2 propelled gene gun (200
> psi, distance 3 cm with 400 mesh nylon screen) using
> tungsten particle (600 nm diameter) coated with plasmid
> expressing anti-ampicillin gene, the plasmid with antiampicillin gene was
> high efficiently transferred into E.
> Coli cells."(
> http://www.sciencepub.net/nature/0204-supplement/03-mahongbao-schistosome.pdf)(3rd
> page)
>
>

> This link <http://physics.ucsd.edu/~groisman/Gene%20guns.html> also has an

> interesting approach to prevent harm by preventing the shock wave of gas
> from directly hitting the culture. If you could get both of these to work a
> smaller portable gun should be possible.
>


--

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