Re: [DIYbio] small-scale metagenomics?

Hi all,

I just saw a mention to this company that supposedly offers 3000 16S pyrotag reads for only $150 (presumably by multiplexing a bunch together on a single 454 run). This might be a great affordable solution to do some small-scale microbial diversity studies:

http://www.researchandtesting.com/documentation-and-submissions.php

Not that I'm looking for yet another project to take on, but I have been itching to do a bit more in the environmental sampling area. Maybe this could be combined with the water sampling project we were discussing for BioCurious?

Anyone know of other vendors that offer fractional NGS runs? It should be feasible to multiplex dozens of transcriptomics samples on a single $2K Illumina run, for example. The hard part for us is being able to find a vendor that bothers to work at such a small scale. But I do think there is a big demand for mid-scale sequencing, somewhere between a couple of Sanger reads, and a full-scale next-gen sequencing run.

Patrik

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