Hi,
as I'll be getting Agrobacterium plasmids quite soon,
and there was that paper that said chloroplast transformation using agrobacterium is feasible,
do you think it would be worth a try?
Basically, I'd use an empty Ti plasmid, PCR a kanamycin resistance and lux operon within it.
I think, I wouldn't even need a promoter etc. because the chloroplasts can express operons. So in some of the transformed chloroplasts the gene cassete is inserted correctly between two genes of an operon.
Am I completely wrong?
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