[DIYbio] Re: Penicillin to "Clean" algae cultures?

Penicillin won't work. It is active vs.  Gram+ and Cyanobacteria are Gram-

There is no perfect antibiotic that kills all bacteria. Even using "powerful" broad spectrum antibiotics eg. chloramphenicol 100ppm you may not remove all the contaminant bacteria from your culture.

I had that problem with some fungal isolates contaminated with bacteria in the lab. I finally used Streptomycin sulphate (15 mg l-1, Sigma), Gentamicin sulphate (15 mg l-1, Sigma) and Tetracycline hydrochloride (12 mg l-1, Sigma). It should work too for your algae. There are other combinations but I have them in the lab. I can check on Monday if you want

El sábado, 17 de noviembre de 2012 03:27:17 UTC+11, Conner Berthold escribió:

Hi,

Was wondering if anyone had tried to use antibiotics to make bacteria free, axenic algal cultures? If found this paper on it, and the main problem looks like the need for a mixed antibiotic mixture to effectively remove the bacteria.

-Conner

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