Re: [DIYbio] Vibrio fischeri isolation

On Thu, Nov 29, 2012 at 12:04 PM, Mega <masterstorm123@gmail.com> wrote:
> Hi, two days ago I bought a piece of Salmon, cut away the skin. I placed the
> skin on a plate and put some salty water (30g/L) on the plate, yet leaving
> the salmon-skin unwetted.
>
> Left it in our basement (some 10-15°C maybe).
>
> As I had a look today, it was amazing. They are very bright. (They are
> nearly as bright as the pVIB E Coli were, althought the Colis had the operon
> ~20 times (medium copy based on pBR322), while V. Fischeri has just a single
> copy of the operon).
>
> You just walk into the room, switch of the light and it glows amazingly
> (i.e. visibly without eye adaption).

I may have missed an earlier discussion on this, but... can you give
more information on what's going on here? It sounds like you just put
fish skin on a plate with saltwater and then it started glowing.

-Dan

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