Re: [DIYbio] 0.2 um filters for sterile filtering/possibly viscous solutions

Almost all PP is autoclavable but may risk slight deformation over a long period of time. At standard autoclave temp of 121 C it will be 9c below polyprop melting point. I sterilize my eppitubes, cutting sheets, etc if i see the PP 5 symbol on the plastic. Tips may deform if your autoclave is not steady at 121.

Sebastian S Cocioba
CEO & Founder
New York Botanics, LLC

Sent via Mobile E-Mail

On Dec 31, 2012, at 11:43 AM, Eugen Leitl <eugen@leitl.org> wrote:

>
> Happy $holidays!
>
> A quick question: I need to sterile-filter several 10 l of a
> reasonably viscous solution into autoclavable borosilicate
> lab bottles.
>
> This is typically done with syringe filters,
>
> http://www.gilsonuk.com/catalogue/product/itemNo/ALG422A
>
> but that's on a too small scale, and for rather nonviscous
> solutions.
>
> I was thinking about repurposing household items like
> http://www.amazon.de/Keramikfilter-Wasserfilter-NEU-Filter-Feinfilter/dp/B002RLKE7O/
> or http://www.amazon.de/Polypropylene-Auswaschbar-UMKEHROSMOSE-WASSERFILTER-SEDIMENTFILTER/dp/B002RLZS7U/
>
> Has any of you done that? How did you sterilize the assembly?
> (Polypropylene is obviously only cold-sterilizable, and
> I suspect the plastic parts of the ceramics one won't
> survive autoclaving either).
>
> At this point I'm thinking about overnight soaking the ceramics
> filter/whole filtering assembly in hydrogen carbonate/hydrogen
> peroxide, 70% isopropanol or bleach, and flushing out the assembly
> for an hour with reverse osmosis water, possibly prefiltered.
>
> Thanks and a nice 2013 to ye all.
>
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