Happy $holidays!
A quick question: I need to sterile-filter several 10 l of a
reasonably viscous solution into autoclavable borosilicate
lab bottles.
This is typically done with syringe filters,
http://www.gilsonuk.com/catalogue/product/itemNo/ALG422A
but that's on a too small scale, and for rather nonviscous
solutions.
I was thinking about repurposing household items like
http://www.amazon.de/Keramikfilter-Wasserfilter-NEU-Filter-Feinfilter/dp/B002RLKE7O/
or http://www.amazon.de/Polypropylene-Auswaschbar-UMKEHROSMOSE-WASSERFILTER-SEDIMENTFILTER/dp/B002RLZS7U/
Has any of you done that? How did you sterilize the assembly?
(Polypropylene is obviously only cold-sterilizable, and
I suspect the plastic parts of the ceramics one won't
survive autoclaving either).
At this point I'm thinking about overnight soaking the ceramics
filter/whole filtering assembly in hydrogen carbonate/hydrogen
peroxide, 70% isopropanol or bleach, and flushing out the assembly
for an hour with reverse osmosis water, possibly prefiltered.
Thanks and a nice 2013 to ye all.
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[DIYbio] 0.2 um filters for sterile filtering/possibly viscous solutions
8:43 AM |
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