Sounds like the main bottleneck with thethe Rapidcycler is the heat transfer from and to the sample - hence the use of glass capillaries and mineral oil. If you're using plastic PCR tubes, you should count on a much longer ramp time. That's probably why the Peltier was more efficient.
Patrik
--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To post to this group, send email to diybio@googlegroups.com.
To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio?hl=en.
To view this discussion on the web visit https://groups.google.com/d/msg/diybio/-/XkeeEEXQ_WUJ.
For more options, visit https://groups.google.com/groups/opt_out.
Re: [DIYbio] First full DNA extraction, PCR, and gel
7:21 AM |
Subscribe to:
Post Comments (Atom)
0 comments:
Post a Comment