On Sat, Dec 8, 2012 at 5:27 PM, Jeswin <phillyj101@gmail.com> wrote:
I run at fixed current, 75V for 20-25 minutes (fresh, and larger gels)
and 50V for 35-45 minutes(older gels that were stored in 4C before
using).
you mention fixed current, but then quote voltage specs. That would mean you control for gel resistance everytime you make a gel.
Controlling for resistance would probably be more precise for getting even gel results between batches, rather just adding X grams or Y ml of electrolyte/buffer stock powder or solution, you would simply measure the resistance of the warm gel solution and titrate/drop in buffer stock until you reach your target.
I reckon a multimeter with a bult-in ohmmeter is cheaper than a decigram/centigram scale (1/10th gram, 1/100th).
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