I run at fixed current, 75V for 20-25 minutes (fresh, and larger gels)
and 50V for 35-45 minutes(older gels that were stored in 4C before
using).
I noticed that too much heat can cause bubbles to form in some gels
which makes it harder to see faint bands.
On Sat, Dec 8, 2012 at 7:45 PM, Avery louie <inactive.e@gmail.com> wrote:
> Thanks for the replies by the way. I appreciate it.
>
> On Dec 8, 2012 7:44 PM, "Avery louie" <inactive.e@gmail.com> wrote:
>>
>> Jeez. Yea.
>>
>> To spill the beans on my current plan (I will have schematics/parts
>> avalible for discussion later), I am working on a cheap, high quality diybio
>> gel supply. Will it get done? Hopefully. Will it have a sweet lasered box?
>> Probably. It may not be totally adjustable though, that is Tbd.
>>
>> --A
>>
>> On Dec 8, 2012 7:26 PM, "Sebastian S. Cocioba" <scocioba@gmail.com> wrote:
>>>
>>> I run a small gibco horizon setup with an old power supply at 25v and
>>> some low amperage (analog readout) for 3hrs. I need a new system :P
>>>
>>> Sebastian S Cocioba
>>> CEO & Founder
>>> New York Botanics, LLC
>>>
>>> Sent via Mobile E-Mail
>>>
>>> On Dec 8, 2012, at 2:53 PM, Dakota <dkotes@gmail.com> wrote:
>>>
>>> Dedicated gel power supply would be sweet!
>>>
>>> I think 120V at 250mA is pretty common
>>>
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Re: [DIYbio] Current and voltage reqs for electrophoresis power supply
5:27 PM |
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