Re: [DIYbio] Re: the diy equipment list?

I've had much the same experience and while used to want a -80 I recently passed up a cheap one as I didn't want the energy expenditure and -20 was working for everything. However I now find myself needing to make a library so need cells of high transformation efficiency and all the commercial cells I find say they require -80. So questions:

1. Anyone have experience storing high competency commercial cells at -20?

2. What does the minimal liquid nitrogen environment look like? I suppose I need a dewar, how long will the liquid nitrogen last? Any tips on what to get?

-Josh

On Mon, Dec 3, 2012 at 9:56 PM, Derek <der...@gmail.com> wrote:

You really don't need a -80 (or -50 even) for most things. I was keeping my strains in liquid nitrogen for the first while and it was cool to have around, but a prof at school told me he kept his E. coli strains in a -20 for all 7 years of his grad school and didn't have any problems. I've got strains that are 4 years old now from my -20 regular chest freezer and are still fine (no defrost cycle, of course.) The only thing I haven't been able to reliably do in the -20 is keep competent cells very good for more than a couple days. I usually just make them as I need them now with the PEG/DMSO protocol unless I need particularly high competency for something.

--Derek

On Monday, 3 December 2012 19:30:35 UTC-8, Nick F wrote:

-50 or lower freezer would be nice, but I imagine shipping on that might be massive, plus I think they need a special dedicated 220V line?  I could be mistaken though.

this can be achieved by replacing the gas with r22 or 134a, I'm having a friend (and expert) researching if a normal freezer can use this with som small mods like better insulation and stuff 

and i really digg your centrifuge part, that is almost cheaper than diy solution ^^ definitely gonna look into this when i get to that point in lab setup

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