They document is attached-thats neat!
Fortunately, you freezer does not heat up too much if it is well insulated and has a lot of thermal mass. And unfortunately, the enzymes in the article look like they were dehydrated and dessicated- most of the enzymes I purchase are already in solution.
Still, it is something to think about for shipping- which can be as expensive as the enzyme itself, if you are not careful.
--A
On Tue, Mar 12, 2013 at 3:46 PM, Phil_ <philgoetz@gmail.com> wrote:
I've been puzzled over how a small lab could store their enzymes. Even in the US, the power goes out for at least a day about once a year. As we "all know", enzymes must be stored at -20F, kept in an ice bucket while you're using them, and returned as soon as possible to the freezer. It would be a major blow to a small lab if all their enzymes were destroyed once a year by a power outage.
This paper claims that all the restriction enzymes they tested maintained their activity at room temperature for a week, and most maintained activity for a full year:
http://www.biotechniques.com/multimedia/archive/00011/00293st06_11772a.pdf
(The file may be attached if that google groups feature really works.)
What do you think? Is it okay to just let your enzymes warm up when the power goes out?
(A related question is: How low a storage temperature can you use on enzymes in a 1:1 glycerol suspension without freezing them? -20 is okay; how about -30?)
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