Re: [DIYbio] Gel Red questions

On Tue, Mar 12, 2013 at 12:57 PM, Nathan McCorkle <nmz787@gmail.com> wrote:
> On Tue, Mar 12, 2013 at 8:42 AM, Jeswin <phillyj101@gmail.com> wrote:
>> Any idea how to use the 3x in water stock gel red? The protocol is here:
>> http://www.biotium.com/product/product_info/Protocol/PI-41001.pdf
>
> It says how to use it in the protocol, on the right middle/lower side.
>
> Either use 3X for post-staining gels, at least 2-3 gels worth of
> post-stains per volume (assuming you need at least enough liquid to
> cover or keep the gel immersed)
> or
> for 60mL gel Use 20mL 3X with 6mL 10X buffer and 34mL dH20
>
>

Hey Nate, I saw that for preparation but how much per gel? 5uL? Is the
1x final solution at 1:10000 dilution? I don't do post staining; only
precasting.

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