Re: [DIYbio] Gel Red questions

On Tue, Mar 12, 2013 at 10:10 AM, Jeswin <phillyj101@gmail.com> wrote:
> Hey Nate, I saw that for preparation but how much per gel? 5uL? Is the
> 1x final solution at 1:10000 dilution? I don't do post staining; only
> precasting.

If you don't do post-staining then you always want the working
concentration to finally be 1X. If the solution starts as *3X*
concentration:
for 60mL gel add: 20mL *3X*, 6mL *10X* buffer and 34mL dH20

If the starting solution is *10000X*
for a 50mL gel:
50mL * 1000uL/mL / 10000X = 5uL *10000X*
49.995 mL 1X buffer

--
-Nathan

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