So we did some troubleshooting, and noticed that the error is very likely that my professor programed the thermcycler to 95°C instead of the 98°C mentioned many times in the description. At that temperature, the antibody from the Phusion polymerase still inhibits its work, we assume.
At the time, he told me that it's just for denaturing, and 95°C would be absolutely sufficient. Seems not.
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