Re: [DIYbio] Re: experienced beginner is creating a bioluminescent plant - both synbio and old-fashioned way

7:33 AM |

Was pVIB the control? Did you do an initial denaturation? Was it a
HotStart- Phusion?

I've been having a lot of trouble with primer dimers myself lately but
after a lot of runs and narrowing things down, finally figured out
that it was poor template DNA (or lack there-of) that caused the
problem. I've never worked with Phusion before, but perhaps that 98C
would make the critical difference.

Keep us posted, cool stuff!

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