Re: [DIYbio] Non-Platinum Electrophoresis Electrodes That Don't Degrade

I have had good luck with stainless steel seizing wire from West Marine. It is $20 for 55 ft.  I often run 150v with TAE and no corrosion of the wire or discoloration of the buffer. 

Roger 

On May 26, 2013, at 2:29 PM, Josiah Zayner <josiah.zayner@gmail.com> wrote:

I have been testing out a bunch of different metals and they either oxidize to death on the minutes timescale or in the case of stainless steel turn the buffer orange/brown.

Platinum wire is pretty expensive ~$1.00 cm^-1. Has anyone had luck with a cheaper metal at 100V or greater with TAE?

I have tried:
Nickel
Stainless Steel 430
Tinned Copper

Thinking of trying Titanium or Nickel Titanium (<= $4 meter^-1). Anyone ever tried these?


Thanks,
     Josiah Zayner


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