Yes that's what I mean. Often a polymerase/PCR kit will come with control DNA and primers. You might need to use different temperature profile, but the point is to test the chemicals and enzymes. So make sure you use all the same water, buffer, nucleotides, etc...
On May 1, 2013 1:47 PM, "Mega" <masterstorm123@gmail.com> wrote:
-- Never heared of that before. You mean e.g. doing also a PCR of e.g. a pUC19 fragment in paralell to make sure the polymerase works?
But that will need different annealing temperatures. In a seperate reaction?
I would have said, if there's a 6.3kbp fragment, the PCR was succesful?
--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio?hl=en.
To view this discussion on the web visit https://groups.google.com/d/msg/diybio/-/irfQn1ovPsAJ.
For more options, visit https://groups.google.com/groups/opt_out.
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio?hl=en.
For more options, visit https://groups.google.com/groups/opt_out.
0 comments:
Post a Comment