Re: [DIYbio] Re: *experienced* beginner is creating a bioluminescent plant - both synbio and old-fashioned way

Never heared of that before. You mean e.g. doing also a PCR of e.g. a pUC19 fragment in paralell to make sure the polymerase works?

But that will need different annealing temperatures. In a seperate reaction?


I would have said, if there's a 6.3kbp fragment, the PCR was succesful?

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