Re: [DIYbio] Re: *experienced* beginner is creating a bioluminescent plant - both synbio and old-fashioned way

On Wed, May 1, 2013 at 7:33 AM, Dakota Hamill <dkotes@gmail.com> wrote:
> Was pVIB the control? Did you do an initial denaturation? Was it a
> HotStart- Phusion?

I second this question, where's the control lane? Control in this case
would not be genomic, it would be a plasmid or linear DNA and primers
KNOWN to work for an amplicon of KNOWN size. This way you can
understand whether your polymerase is actually doing anything.

--
-Nathan

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