top band is a ladder, the largest band is 1.5 kb, and the smallest is 100 bp. the two lanes beneath it are samples. the gel was run for about an hour and a half at 60 v and about 50 ma.
here is a photo of the gel!
On Jul 15, 2013 11:14 PM, "Avery louie" <inactive.e@gmail.com> wrote:hello all,
i ran a rather silly gel today. i wanted to see if a double digest had worked, but the fragments should be about 6 bp and 4kb! so i obviously was not able to resolve both on my mini gel.
i saw a very large fragment, probably around 4 kb on the gel. if it were plasmid dna, i would expect it to run as a smaller fragment. it seems to have worked, but i want to check my reasoning with you all. obviously, this does not prove both digests worked, but it at least suggests that one did.
if i really needed to make sure it was digested, i would run control single cut reactions against plasmid dna, and the double digest, but i dont have a ton of template dna.
--Avery
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