here is a photo of the gel!
On Jul 15, 2013 11:14 PM, "Avery louie" <inactive.e@gmail.com> wrote:
-- hello all,
i ran a rather silly gel today. i wanted to see if a double digest had worked, but the fragments should be about 6 bp and 4kb! so i obviously was not able to resolve both on my mini gel.
i saw a very large fragment, probably around 4 kb on the gel. if it were plasmid dna, i would expect it to run as a smaller fragment. it seems to have worked, but i want to check my reasoning with you all. obviously, this does not prove both digests worked, but it at least suggests that one did.
if i really needed to make sure it was digested, i would run control single cut reactions against plasmid dna, and the double digest, but i dont have a ton of template dna.
--Avery
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/CAL4KOmg_sbaDuv_8dsPX8vYVPGawesOy8QrXLSNQrx6L3QbRUA%40mail.gmail.com.
For more options, visit https://groups.google.com/groups/opt_out.
0 comments:
Post a Comment