[DIYbio] restriction digest confirmation

hello all,

i ran a rather silly gel today.  i wanted to see if a double digest had worked, but the fragments should be about 6 bp and 4kb!  so i obviously was not able to resolve both on my mini gel.

i saw a very large fragment, probably around 4 kb on the gel.  if it were plasmid dna, i would expect it to run as a smaller fragment.  it seems to have worked, but i want to check my reasoning with you all.  obviously, this does not prove both digests worked, but it at least suggests that one did.

if i really needed to make sure it was digested, i would run control single cut reactions against plasmid dna, and the double digest, but i dont have a ton of template dna.

--Avery

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