Re: [DIYbio] Re: restriction digest confirmation

On Tue, Jul 16, 2013 at 12:37 PM, Nathan McCorkle <nmz787@gmail.com> wrote:
> So just do the math to bump the fragment up to the minimum detection
> concentration by adding more template... or after digestion, ligate on
> adapters (primers) to bump up the mass of ANY/ALL fragments. In the
> latter case, you'd then need to differentiate 6bp.
>
> (formula_weight_of_nucleotide*length_of_DNA_in_band) *
> (concentration_of_limiting_reagent*microliters_of_reagent_added/num_microliters_in_liter)
> / num_bands_expected * num_nanograms_in_gram = nanograms_DNA_in_band
> (500*6)*((3*10^-6)*1/1000000)/100*10^9
> =0.09 nanograms
>
> this is assuming your pre-digested plasmid concentration was 3
> millimolar,

sorry micromolar, not milli!!!!

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