Why wouldn't you use flame sterilization? It doesn't require any special equipment, just clean burning flammable liquid and a lighter. Just seems stupid to do this sort of thing without taking the simple steps like that. If you're going to post labbook-esque blog posts, you should include a better list of materials, components of you buffers, etc. This isn't just an issue I have with you, but a lot of the posts in diybio have some results without anything that goes with it, their procedures and materials, it really doesn't help further the community to just show what cool thing you've done but give half the details.
On Tue, Aug 6, 2013 at 11:39 PM, jarlemag <jarle.pahr@gmail.com> wrote:
Cool!
What concentration of chloramphenicol did you use?
-JP
kl. 05:37:20 UTC+2 onsdag 7. august 2013 skrev Dakota følgende:To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/8ea14c1b-5ecd-4d44-9977-ad7c1d2fa7dc%40googlegroups.com.Hi all, just wanted to share some encouraging results from a few recent experiments we've undertaken, specifically, the isolation of symbiotic endophytic fungus without a laminar flow hood and expensive equipment, followed by sequencing data from a PCR of a common button mushroom purchased for 25 cents from the grocery store. The PCR and sequencing was basically a "validation" of our DNA isolation protocol, PCR program, cleanup kit, and sample submission, with the goal that it will be used to ID novel fungal strains isolated from plant tissue in the future.--It's pretty cool to get your first chromatogram and have them all be tall skinny peaks!FASTA sequences are in the post for BLASTing if you so desire.-Dakota
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