Re: [DIYbio] Endophyte isolation and first successful sequencing

Hi all, just wanted to share some encouraging results from a few recent experiments we've undertaken, specifically, the isolation of symbiotic endophytic fungus without a laminar flow hood and expensive equipment, followed by sequencing data from a PCR of a common button mushroom purchased for 25 cents from the grocery store. The PCR and sequencing was basically a "validation" of our DNA isolation protocol, PCR program, cleanup kit, and sample submission, with the goal that it will be used to ID novel fungal strains isolated from plant tissue in the future.  

http://basementbiotech.org/basic-endophyte-isolation-testing-the-lower-limits-of-aseptic-technique/

http://basementbiotech.org/down-n-dirty-sequencing/

It's pretty cool to get your first chromatogram and have them all be tall skinny peaks!

FASTA sequences are in the post for BLASTing if you so desire.

-Dakota