Re: [DIYbio] Re: DIY targeted evolution

I'm not sure I understand why you need nitrous acid, but just FYI,
nitrous acid can only be made in situ (you can't buy a bottle of
nitrous acid). You make it in solution by mixing sodium nitrite and
HCl in an ice bath. Also, the nitrous acid only persists in solution
at temperatures close to 0C. Once you heat it up to room temperature
it will decompose into some nasty gases.

Also, a long time ago I was doing some diazonium chemistry (diazoniums
are made with nitrous acid) and I think I remember there being a
maximum concentration that nitrous acid can be at before it
decomposes, even at 0C. I think it was something like 200mM. So
that's something to keep in mind.

If you're just trying to grow bacteria on mutation-inducing media I
would recommend ethyl methanesulfonate instead.


-cory

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