-JP
kl. 06:41:23 UTC+2 søndag 18. august 2013 skrev Nathan McCorkle følgende:
On Aug 17, 2013 12:53 PM, "Jeswin" <phill...@gmail.com> wrote:
>
> The time from 4C to 42C in the regular water bath method is
> insignificant.Your comment later about ramp rate means it /is/ significant.
Some think the thermal shock creates a pressure wave.
The NEB competent cells I work with are only held at
> 42C for 30s. My guess is that the ramp time on a PCR machine is
> significant compared with the time held at 42C. From what I recall of
> the theory, the sudden shock of 42C opens up the pores allowing DNA to
> enter. Slowly ramping probably negates the heat-shock effect.
>
> On Sat, Aug 17, 2013 at 10:36 AM, John Griessen <jo...@industromatic.com> wrote:
> > On 08/17/2013 12:17 AM, Avery louie wrote:
> >>
> >> @nathan, what do you mean? I can probably mathify you.
> >
> >
> > It all depends on the heat conductivity of the vial.
> >
> > Thin vials with agitation --> fast heat ramps.
> >
> >
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