RE: [DIYbio] Thermal Cycler Based Bacterial Trasformation

Ah, I thought it was a range due to the machines ability to set the
actual ramp rate. Didn't know the PCRs have different thermal rates but
now that I think about it it does make a ton of sense.

Sebastian S. Cocioba
CEO & Founder
New York Botanics, LLC
Plant Biotech R&D From: Nathan McCorkle
Sent: 8/20/2013 6:10 PM
To: diybio
Subject: Re: [DIYbio] Thermal Cycler Based Bacterial Trasformation
> From: Keoni Gandall
> Sent: 8/20/2013 1:55 PM
> To: Sebastian Cocioba
> Subject: Re: [DIYbio] Thermal Cycler Based Bacterial Trasformation
> The manufacture says the ramp time is .1C to 5C a second, which I have to go
> with 5C since the machine is like almost new

I believe the lower ramp rate (0.1C/sec) is going from Hot to Cold,
while the faster ramp rate (5C/sec) is going from Cold to Hot. You
might re-read the manual to see if that's mentioned somewhere, but if
it's a peltier-based device, that's likely what these two numbers
mean.

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