Re: Fwd: [DIYbio] Thermal Cycler Based Bacterial Trasformation

The time from 4C to 42C in the regular water bath method is
insignificant. The NEB competent cells I work with are only held at
42C for 30s. My guess is that the ramp time on a PCR machine is
significant compared with the time held at 42C. From what I recall of
the theory, the sudden shock of 42C opens up the pores allowing DNA to
enter. Slowly ramping probably negates the heat-shock effect.

On Sat, Aug 17, 2013 at 10:36 AM, John Griessen <john@industromatic.com> wrote:
> On 08/17/2013 12:17 AM, Avery louie wrote:
>>
>> @nathan, what do you mean? I can probably mathify you.
>
>
> It all depends on the heat conductivity of the vial.
>
> Thin vials with agitation --> fast heat ramps.
>
>
> --
> -- You received this message because you are subscribed to the Google Groups
> DIYbio group. To post to this group, send email to diybio@googlegroups.com.
> To unsubscribe from this group, send email to
> diybio+unsubscribe@googlegroups.com. For more options, visit this group at
> https://groups.google.com/d/forum/diybio?hl=en
> Learn more at www.diybio.org
> --- You received this message because you are subscribed to the Google
> Groups "DIYbio" group.
> To unsubscribe from this group and stop receiving emails from it, send an
> email to diybio+unsubscribe@googlegroups.com.
> To post to this group, send email to diybio@googlegroups.com.
> Visit this group at http://groups.google.com/group/diybio.
> To view this discussion on the web visit
> https://groups.google.com/d/msgid/diybio/520F8A72.3090408%40industromatic.com.
>
> For more options, visit https://groups.google.com/groups/opt_out.

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/CAAhF0RLr6Z8pELeRVdBskKXug%3Do8aTfXBpLF2rcvWeOK7Wuv%2Bw%40mail.gmail.com.
For more options, visit https://groups.google.com/groups/opt_out.