Re: [DIYbio] large dna extraction

On Tue, Feb 25, 2014 at 9:42 AM, Koeng <koeng101@gmail.com> wrote:
> Do you need to get intact DNA? If not I usually just boil cells for a while to get DNA for pcr, which is pretty lazy but works quite well
>
It's for sequencing.

Well, it turns out that the Qiagen Qiaex II kit (beads) to purify the
plasmid didn't give me any dna. Maybe the beads are too old. The gdna
that I collected off the gel (barely went into the gel) was purified
with the chargeswitch kit and gave a good amount of dna. Since we have
gdna, we can sequence the gdna and the total dna. Then, by
subtracting, we can determine the plasmid sequence. That's the plan,
anyway.

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