Re: [DIYbio] Re: Ultra-Cheap DNA Printing/Sequencing

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If you could ship DNA at 1/100 the costs I pay, I wouldn't care if it
had a 1/1000 error rate. That'd probably be my threshold for
correctional PCR, but at 1/100 the cost I could easily afford sequencing
and a few primers to amend errors.

Mind you, I'd still prefer if you'd do the QA at your end, and I only
pay 1/10 the price instead of 1/100, but someone could always operate a
middle-woman service to that effect and I could buy through them.

For me, DNA synth costs are a huge, huge factor in my ability to work,
so that'd be a huge change.

On 25/02/14 21:27, Nathan McCorkle wrote:
> On Feb 25, 2014 8:14 AM, "SC" <stacy734@yahoo.com> wrote:
>>
>>> I'm literally saying I want to do this to pay off my school loans
>>
>> I have huge respect for anyone who starts a business to pay off school
> loans. As a practical matter, I'm not sure this is the way to go. People
> that need synthetic DNA made for them typically don't have the resources to
> test it themselves, so you would have to do QA
>
> The proof is in the pudding, though, if I sent Cathal/mega/Sebastian a vial
> of liquid... Any of them would quickly be able to if it coded for whatever
> they wanted. They'd be doing characterization on the DNA from their
> colonies anyway... PCR, restriction digests, and western blots are very
> common tools in any experiment trying to prove they cloned something or
> prove that something is expressed. Thesr are standard molbio tools, nothing
> new, nothing a decent cloning lab won't have. The only thing uncommon in
> diybio is the western blots, but that's why you just rely on a complex
> reporter system to act like a 'physical hash' of the DNA. If it smells like
> bananas and fluoresces, the DNA was good. Determining percent correct
> strands would be a bit more challenging, but again nothing a lab in any
> large city doesn't already perform everday (cytometry). Cytometry is pretty
> cheap sample wise, and most hospitals have a lab.
>
> before shipping product. More to the point, you would have to convince
> customers you could do QA properly and were reliable. Your end product is
> someone else's starting component for a more complex process, and if it's
> contaminated with incomplete strands (for example), your customers will
> waste time and resources and won't be happy.
>>
>> As synthetic DNA isn't that expensive to buy from well established labs,
> I'm not sure that
>
> Really? You're joking right? I'm talking about orders of magnitude cheaper
> than current markets, my initial goal is 100x cheaper, but my rough
> calculations for downscaling the reaction even further (and still keeping
> overall reaction yield low) show 10^12 cost reduction with a 1micron cube
> reaction center. <=$1000 genome
>
> type of home business would be able to compete effectively in the current
> marketplace. As a consumer of such things, I'd probably go with a better
> known supplier that came with recommendations from my colleagues rather
> than save a little money dealing with a home lab. I don't mean to
> discourage you, so please just consider this a bit of marketing information
> from your target group.
>
> No it's good to hear that when I get it right people's minds will be blown.
>
>>
>> However, if you're doing this for the spirit of invention and don't need
> to generate an income form it right away, I wish you the best of luck.
>
> Thanks, I was lucky to start studying on this my first year of college, and
> its been my goal since then. That was 2008, and I feel much more confident
> these days. Lots of engineering tricks have been accumulating in that time,
> lots of business networking, lots and lots of research and mostly
> instrumentation development.
>
> Luckily I made friends with a guy who has a FIB and underused microfab
> capable wet lab... So things are almost in place for me to make so leaping
> progress in the coming months.
>
>>
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