[DIYbio] Re: Need yeast shuttle vector

I would just email the lab directly. If you pay the shipping I can't imagine a reasonable lab not sending you a vector. This is usually the way it is done in professional circles.

Also, since most of the plasmids on addgene have been published on they are public domain anyway. I always wonder if their MTA is even possible to uphold?

#1 How could anyone possibly determine if the plasmid you used is from addgene?
#2 If the plasmids are published on and public domain, doesn't that negate the MTA regardless?

I don't know much about this legal stuff so...

I think addgene are huge dicks because they sell plasmids for ridiculous amounts after they acquire it for free from a lab.


On Monday, June 2, 2014 1:40:32 AM UTC-7, Marc Juul wrote:

I am part of the Counter Culture Labs crew in Oakland, California, and we're looking for a yeast shuttle vector, preferably with some or all of the following features:

  * URA3 gene
  * Ampicillin resistance gene
  * 2-micro ori

  * An E. coli ori like pUC, pBR322 or f1

  * Alpha factor secretion signal

We realized that we can't use addgene.org since their materials transfer agreement would put limits on our use and sharing of constructs.

Anyone have an IP and MTA unencumbered shuttle vector we can have?

--

marc/juul