Hi Marc,
I've got YEp-24 available. It has everything your looking for except for the alpha factor secretion signal. I can send you an E. coli culture with the plasmid next week if that will work for you.
Tom
BUGSS
On Monday, June 2, 2014 4:40:32 AM UTC-4, Marc Juul wrote:
On Monday, June 2, 2014 4:40:32 AM UTC-4, Marc Juul wrote:
I am part of the Counter Culture Labs crew in Oakland, California, and we're looking for a yeast shuttle vector, preferably with some or all of the following features:
* URA3 gene
* Ampicillin resistance gene
* 2-micro ori* An E. coli ori like pUC, pBR322 or f1* Alpha factor secretion signalWe realized that we can't use addgene.org since their materials transfer agreement would put limits on our use and sharing of constructs.
Anyone have an IP and MTA unencumbered shuttle vector we can have?
--marc/juul
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