Re: [DIYbio] automated fluorescent microscope

I'm working on this too, found the openlabtools data a few months ago
and ordered what should be an appropriate tube lens for monochromatic
use. Already have some infinity objectives from my current fluoro
'scope.

Personally I don't think the RasPi camera is too great for image
quality, but I'll be using it for it's high frame-rate for doing live
object tracking, position tracking, maybe other stuff. For getting
nice images to share with the world, best to find a different camera.
The digital noise on the RasPi camera is very appreciable, most
apparently under low-light conditions.

Why do you want to start with fluorescense? Is there anything you
actually want to see using this? It might be more interesting, if you
don't have a project that specifically requires fluorescense, is
building a monochromator light source. That way you can get full-color
images (of non-moving objects) but also get around chromatic
aberration. Or you can just get monochrome images of moving stuff. The
point is, getting around chromatic aberration is going to cost a few
hundred in lenses, I think.

On Fri, Jun 27, 2014 at 3:30 AM, Michael Shamberger
<shambergerm@gmail.com> wrote:
> Hi,
>
> I am interested in building an automated microscope that would be useful for
> synthetic biology. Features would include automated tracking and
> classification of individual live bacteria. Videos of the time lapse images
> would be transmitted and assembled in a PC and post processed with machine
> learning techniques. I am a computer engineer so the robotics and machine
> learning are in my domain. I want to understand the design for a DIY
> microscope in the sub 1000 range that would have the capability of creating
> the images.
>
> From what I have been researching on the web, I was thinking to build a LED
> Fluorescent microscope. I am starting from the microscope design at
> openlabtools.org. It uses a Rasberry pi and 5MB raspberry pi camera with an
> infinity optical system. I want to add a fluorescent stage to that. There
> are some DIY articles about LED Fluorescent microscopes but are lacking
> details though I continue to search. If someone could point me in the right
> direction I would appreciate it. I need information about where to source
> low cost LED's and design.
>
> Mike
>
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-Nathan

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