Re: [DIYbio] Freezing cells with plasmids

I once smashed e coli + pVIB in the -20 freezer and successfully ressurected them on fresh LB Amp after 4 months.

I think I froze just in sterile water, LB and with glycerol added. (got a little bit contamination with glycerol though on the amp plates, but 80% were e glowli)

maybe ny dirty procedure killed 99% of the cells but there were like 10000000 cells initially so I didn't care

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