Yes up until you have saturated the pores of the resin. These can be washed out to some extent, but will eventually be saturated. Unless you load heavily with unclarified supernatant, you will get many uses of each column.
>matt
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Andreas Stuermer <masterstorm123@gmail.com> wrote:
Probably by just running deionized water through them?
On Sun, Aug 3, 2014 at 9:00 PM, Andreas Stuermer <masterstorm123@gmail.com> wrote:
I ask ebay for "prepacked columns of sepharose"
Can u reuse them?--
On Sun, Aug 3, 2014 at 8:59 PM, Andreas Stuermer <masterstorm123@gmail.com> wrote:
Thanks!!
http://www.piercenet.com/product/disposable-plastic-columns
These also seem nice. Easy to use?--
On Sun, Aug 3, 2014 at 8:55 PM, Matt Lawes <matt@insysx.com> wrote:
--Chromatography column is the appropriate term. Typically glass or plastic. You may even find prepacked columns of sepharose / sephadex. Go to GE healthcare life sciences and look at sepharose / sephadex technical info to determine which resin (size exclusion) will work best for you.>matt
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Andreas Stuermer <masterstorm123@gmail.com> wrote:
You received this message because you are subscribed to the Google Groups "DIYbio" group.Gravity flow sepharose sounds like a way to go. Have you got a keyword how I can find a column where to put in the Sepharose?--
On Sun, Aug 3, 2014 at 8:39 PM, Josiah Zayner <josiah.zayner@gmail.com> wrote:
Gravity flow size exclusion should work fine. As someone else said use Sepharose or Sephadex. You can find it on eBay pretty cheap. If you have money to spare buying old (H)FPLC pumps is pretty cheap on eBay. I think there are people who do (H)FPLC on this list but people probably just don't have time or motivation to run samples for you, including me.
As Cathal said, An ammonium sulfate precipitation is a tried method that has been used for over 60? years. By increasing.decreasing the ammonium sulfate concentration you can precipitate out proteins and try the different fractions.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/bd782b26-3dba-4ada-8794-a848a0615cc2%40googlegroups.com.
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