-----BEGIN PGP PUBLIC KEY BLOCK-----
Version: GnuPG v1.4.12 (GNU/Linux)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=wOG4
-----END PGP PUBLIC KEY BLOCK-----
-----BEGIN PGP SIGNATURE-----
Version: GnuPG v1.4.12 (GNU/Linux)
Comment: Using GnuPG with Thunderbird - http://www.enigmail.net/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=bgVj
-----END PGP SIGNATURE-----
Can someone tell me what the difference is between sepharose and
agarose? Is sepharose agarose beads with known porosity for
chromatography, or is it just plain agarose, or chemically modified agarose?
I recall during research into nickel columns that sepharose was at least
based heavily on agarose with a few tiny changes, but I don't know
whether those changes were specific to the nickel immobilised version of
sepharose, or general to "sepharose", nor do I know in the latter case
what the changes accomplish..
On 03/08/14 19:39, Josiah Zayner wrote:
> Gravity flow size exclusion should work fine. As someone else said use Sepharose or Sephadex. You can find it on eBay pretty cheap. If you have money to spare buying old (H)FPLC pumps is pretty cheap on eBay. I think there are people who do (H)FPLC on this list but people probably just don't have time or motivation to run samples for you, including me.
>
> As Cathal said, An ammonium sulfate precipitation is a tried method that has been used for over 60? years. By increasing.decreasing the ammonium sulfate concentration you can precipitate out proteins and try the different fractions.
>
--
T: @onetruecathal, @IndieBBDNA
P: +353876363185
W: http://indiebiotech.com
Re: [DIYbio] DIY Protein Separation
12:23 PM |
Subscribe to:
Post Comments (Atom)
0 comments:
Post a Comment