[DIYbio] Re: help me setup DIY-BIO home Lab to produce Recombinant Factor VIII

Hi Sunil.  I admire your goal, but your questions are something akin to "How can I perform surgery? And can someone send me a scalpel?"

There are lots of very basic skills you have to master before you can jump into a complex project like this.   For example, working with a sterile cell culture, making recombinant anything (and you shouldn't start with eukaryotes for this, really), and protein purification.  Start slow. Learn the basics.  And read up on immunology.  Also, these procedures can be expensive, especially growing mammalian cells; you can't do it with a light bulb and a shoebox that one might get away with for bacterial cells.   You need to have an understanding of the equipment and supplies you may need, and a realistic budget.

I truly wish you all the best for your project.

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/8c5535ac-2939-44b1-88d4-069e3a5e1658%40googlegroups.com.
For more options, visit https://groups.google.com/d/optout.

  • Digg
  • Del.icio.us
  • StumbleUpon
  • Reddit
  • RSS

0 comments:

Post a Comment