Re: [DIYbio] Re: help me setup DIY-BIO home Lab to produce Recombinant Factor VIII

On Fri, Jan 30, 2015 at 10:41 AM, 'SC' via DIYbio
<diybio@googlegroups.com> wrote:
> Also, these procedures can be
> expensive, especially growing mammalian cells; you can't do it with a light
> bulb and a shoebox that one might get away with for bacterial cells.

Well I'd argue that you can grow mammalian cells on-the-cheap, but
you've got to be a real expert at this stage because no one has laid
out an instructables or kit-type protocol for this.

Personally I've been wanting to grow some pig (porcine) heart cells
using locally-sourced reagents (i.e. spice shops, grocery stores, and
pharmacies)... and I think it isn't too outlandish... but definitely
time consuming to source a media mixture from mixed 'off-brand'
sources and also rig up lab equipment (petris, reagents, centrifuge
for grabbing plasma from blood, keeping things everything sterile and
clean) at a slaughterhouse/farm-barn. DIY-able, sure, but not as a
beginner unless you're a savant or genius or insanely
dedicated/driven.

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