Re: [DIYbio] Re: Is it possible for a Homeworker to create a thermosensitive yoghurt?

1:31 PM |

Hi Lukas,

it might actually be possible. I found a fluorescent protein that experiences an absorbance shift in a range between 4°C and 50°C.

Take a look here: http://www.nature.com/srep/2012/120829/srep00608/full/srep00608.html

The protein that is used in that study is called WasCFP (derived from mCerulean by mutagenesis). You might be able to transform your bacteria with a plasmid carrying a gene for that protein. Then you might be able to produce a heat sensitive yoghurt. ;)

I did not take a very close look into it yet, but that might be the right direction.

Cheers
Filip


2015-01-28 21:42 GMT+01:00 Mega [Andreas Stuermer] <masterstorm123@gmail.com>:
Why do you want to add external luciferin? In bacteria, the vibrio fischeri lux system works pretty well and does not require external substrates

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