Re: [DIYbio] Re: low cost positive pressure laminar flow HEPA filtered box

I've had good success with a simple positive pressure glovebox with a
large HEPA filter for large rooms; high enough pressure that tools would
get blown out of the work area, in fact.

It worked great, no contaminations.. until the HEPA filter saturated on
mold spore in an old building where I hosted workshops, and that was
that. I never replaced the filter as I had little need; I'd secured a
bunsen and a safe work-space on which to light it.

On 28/01/15 17:31, Ilya Levantis wrote:
> I'm extremely dubious of set ups using the air purifier HEPAs. I managed
> to get a specification and test sheet from a typical supplier of those
> filters (https://www.dropbox.com/s/1i7qo8v6xgxwov7/DOP%20test.pdf) and
> it suggests to me you would never be able to get laminar flow going from
> one of those - you would probably need some huge fan or an array of
> leafblowers to actually push enough air through them, and by that point
> the actual particle catching efficiency would probably not be as effective.
>
> But some people appear to be having success with such setups - I'm
> curious to know more.
>
> On Wednesday, 28 January 2015 16:25:40 UTC, Cathal Garvey wrote:
>
> I can't imagine "lint free" can be guaranteed enough for industrial
> standard with a sheet, either. At least, not with a sheet that's
> cheaper
> than a large HEPA filter in the first place.
>
> On 28/01/15 15:07, John Griessen wrote:
> > On 01/28/2015 07:57 AM, Cathal Garvey wrote:
> >> Could you mimic this though, by passing air through a HEPA and then
> >> having it (inside the sterile space) pass through a lint-free,
> >> pre-sterilised fabric sheet?
> >
> > Yes, and that would protect your filter from splashes also. Muslin
> > might be right for it, or maybe cheesecloth...
> > The filter still needs to flow slowly enough to work well,
> > so it still needs to be large enough...just how large is probably a
> > measure-test-repeat-while-necessary design process though.
> >
> > Fabric must seem too "homey" to be an industry standard. The
> industry
> > standard way you see in ads is a filter,
> > then a plenum, (or manifold), then a panel with slots to release
> > the air. The panel is the full size of the back of the
> rectangular box
> > of the flow hood. I'm sure some kind
> > of stretched fabric could do even better at laminar flow than a
> panel
> > with slots.
> >
> > Probably the wall of slots is the best compromise between needing to
> > "change the sheets" often, and having a little
> > dust left after wiping down the front surface of the wall of slots.
> > Since we think of a flow bench as durable,
> > there needs to be a way to clean it.
> >
>
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