Re: [DIYbio] Re: low cost positive pressure laminar flow HEPA filtered box

On 01/28/2015 07:57 AM, Cathal Garvey wrote:
> Could you mimic this though, by passing air through a HEPA and then having it (inside the sterile space) pass through a lint-free,
> pre-sterilised fabric sheet?

Yes, and that would protect your filter from splashes also. Muslin might be right for it, or maybe cheesecloth...
The filter still needs to flow slowly enough to work well,
so it still needs to be large enough...just how large is probably a measure-test-repeat-while-necessary design process though.

Fabric must seem too "homey" to be an industry standard. The industry standard way you see in ads is a filter,
then a plenum, (or manifold), then a panel with slots to release
the air. The panel is the full size of the back of the rectangular box of the flow hood. I'm sure some kind
of stretched fabric could do even better at laminar flow than a panel with slots.

Probably the wall of slots is the best compromise between needing to "change the sheets" often, and having a little
dust left after wiping down the front surface of the wall of slots. Since we think of a flow bench as durable,
there needs to be a way to clean it.

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