I would love if you could fix/update these issues/points that I've
found, then upload the new documents to a public URL so I can post
links to Facebook, etc...:
"The exhaust gas will pass through the reaction chamber and the SOx
and NOx in the gas stream will be reduced by the bacteria."
-- what exhaust gas??? you haven't mentioned.
"Our team planned to reduce the amount of the NOx and SOx gases
ejected through the exhaust vents."
-- what exhaust vents?
"positive zeta potential"
-- you should give a quick explanation of this, I am a scientist by
training and an engineer by day (software/electrical these days) but I
don't know that term... any rich business people that aren't
scientists/engineers will definitely not know what this is, and
probably won't be inclined to google the term.
what was your previous success, how about efficiency/completeness of
the gas cleanup?
what are SRCs?
"The genetically engineered bacteria will be immobilized onto
reactors, and will be used to reduce the pollution from diesel
generators."
-- only in slide 8 do you state what should be the second sentence of
the entire slide deck (and the main starting point of the PDF)
-- In the PDF you say:
'Working on the "Eco.coli" , we have managed to succesfully
synthesize the bacterial clone capable of reducing NOx and SOx into
NH3 and sulfur respectively. However, due to lack of time and funding,
we could not develop the table top model last year.'
-- but then in the powerpoint slides you mention that the Cysl gene
has not been cloned yet, and that in fact you only created the Nrfa
and Sqr clones and got them sequenced, it doesn't mention anything
about gas-stream remediation experiments. This is misleading, and
could cause some people to lose trust in your team or the project. Be
more clear about previous accomplishments, current status, and
roadmap/plan moving forward (should you get the $$$).
P.S. is this the gene system that normally functions in plant-root
ecosystems, and is generally anoxic (poisoned by oxygen)?
On Sat, May 30, 2015 at 10:35 AM, Ujjwal Thaakar
<ujjwalthaakar@gmail.com> wrote:
> Hi,
> My friends at IIT Delhi, India want to go to iGem. They won the bronze medal
> last year at iGem Jamboree 2014. Unfortunately they haven't found very many
> sponsors yet..
>
> They're aiming for the gold this time with a portable system that uses
> genetically modified bacteria to consume air pollutants and toxics, thus
> purifying the air. They need $16K. How do you guys suggest we raise this
> money. Can we as a community help them move forward by pooling in some
> money? If everyone on this mailing list even donates $4 then we'll meet the
> goal and help them go ahead.
>
> I'm attaching a PPT and the sponsorship prospectus if someone if interested.
>
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-Nathan
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Re: [DIYbio] Sponsorship & Raising money for iGem
[DIYbio] Sponsorship & Raising money for iGem
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Re: [DIYbio] Re: Will this sequence work as promotor?
Am Samstag, 30. Mai 2015 17:42:34 UTC+3 schrieb Michael Tellier:
Interestingly, most Salmonella genomes have lost the lac operon, which seems to hinder its pathogenicity by down-regulating the genes involved in the flagelles. So if you put your construct in Salmonella, your gene will be always expressed since LacI is likely to be absent. You might need to add the LacI gene on your construct to express the repressor.About Salmonella and the absence of the lac operon:http://link.springer.com/article/10.1007%2Fs00284-014- 0720-7
On Saturday, 30 May 2015 15:02:00 UTC+1, Mega [Andreas Stuermer] wrote:E coli and Salmonella are more or less related. So I guess the coli promoter woud likely work without modification. Does Salmonella have a lac repressor?
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Re: [DIYbio] Re: Will this sequence work as promotor?
Am Samstag, 30. Mai 2015 17:02:00 UTC+3 schrieb Mega [Andreas Stuermer]:
E coli and Salmonella are more or less related. So I guess the coli promoter woud likely work without modification. Does Salmonella have a lac repressor?
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Re: [DIYbio] Re: Will this sequence work as promotor?
On Saturday, 30 May 2015 15:02:00 UTC+1, Mega [Andreas Stuermer] wrote:
E coli and Salmonella are more or less related. So I guess the coli promoter woud likely work without modification. Does Salmonella have a lac repressor?
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Re: [DIYbio] Re: Will this sequence work as promotor?
E coli and Salmonella are more or less related. So I guess the coli promoter woud likely work without modification. Does Salmonella have a lac repressor?
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[DIYbio] Cultivation of bacteria
Hi everybody!
I wanted to learn the basics of DIY-Bio and so I started to cultivate bacteria that grow on my hand. After my 4th attempt there is still no result. I used gelatine and distilled water.
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Re: [DIYbio] Re: Will this sequence work as promotor?
Extremely hard to say... I just know from T7 if you space lacO (or was it tetO) more than 5 bp away it doesn't work anymore because the DNA helix. The repressor will bind the operator but in a different angle... Have you made sure that the core promoter will be in the same position relative to the operator? Isn't it possible to take the native coli one?--
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[DIYbio] Re: Will this sequence work as promotor?
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[DIYbio] Will this sequence work as promotor?
It is based on p22 Salmonella phage promotor with replacement of OR1 and OR3 repressor binding sites by lactose operon repressor binding sites from Escherichia coli. How probable is what this construction will work?
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[DIYbio] Re: Anyone in the Sacramento/Davis area interested in making a bio hackerspace?
I'm starting a PhD at UC Davis this fall, and yes, I'm definitely interested. I don't have a really clear idea of how to go about doing it, though.
> On Dec 21, 2011, at 10:49 PM, CodonAUG <elsbernd@gmail.com> wrote:
>
> Is there anyone in the Sacramento/Davis area interested in making a
> bio hackerspace (something akin to GenSpace or BioCurious).
>
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Required || WebSphere Administrator || Dubuque, IA || 12+ Months || Phone And Skype || Only USC/GC
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WebSphere Administrator
Dubuque, IA
12+ Months
Phone And Skype
Only USC/GC
Required
Middleware Administration experience with WebSphere Application Server, BEA Weblogic Application Server, IBM HTTP Server/Apache, Sun Web Server, and/or WebSphere; support within operating systems and Web Middleware infrastructure environments; Middleware Administration experience within WebMethods, Tibco, BizTalk, Weblogic and/or Cyclone Band 5 - Server Systems Operations - Application Hosting Services - Web Middleware Enablement - WebSphere Application Server. Systems Management Specialist
Thanks and Regards,
Arpit Arora | Sr.Technical Recruiter
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Required || .Net Developer || Lansing,MI || 12+ Months || USC / GC Only || Phone And Skype
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.Net Developer
Lansing, MI
12+ Months
USC / GC Only
Phone And Skype
Required
Experience Required:
At least five years' experience with web development in ASP.Net and C#
At least five years' experience with web development in JavaScript
At least three years' experience with web development and an Oracle or SQL Server backend
At least six years' experience in object oriented programming
At least two years' experience with PL/SQL
At least three years of experience with Microsoft's MVC 4.0 or higher framework, including razor. Application Developer C#.NET
ASP.NET, C# 4.x, MVC Razor 5, HTML 5, CSS3, JQuery, JavaScript, Oracle and SQL Server database, object oriented development, PL/SQL, Test Driven Development, Unit Testing Familiarity With: JSON, Bootstrap, LINQPAD, Refactoring, MOQ, Design Patterns, Clean Code, Fiddler, IE Developer Tools, Fluent, Ninject, GRIDMVC
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Technology Resource Group Inc.
3736 Hillsdale Court Santa Clara, CA 95051
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Required || SSIS/ETL Developer || San Antonio, TX || 12+ Months || Phone And Skype || USC / GC
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SSIS/ETL Developer
San Antonio, TX
12 months Contract
US Citizen and Green Card holders
Client prefers local candidates.
Position Overview
· SSIS Developer with Extremely strong skills with ETL
· They are in a Parallel data warehouse (Microsoft APS)
· Nice to have: PowerShell / python/ Hadoop/Apache
· All Microsoft technologies
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Arpit Arora | Sr.Technical Recruiter
Technology Resource Group Inc.
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[DIYbio] Re: Anyone in the Sacramento/Davis area interested in making a bio hackerspace?
http://www.meetup.com/BeniciaMakerspace/
Is there anyone in the Sacramento/Davis area interested in making a
bio hackerspace (something akin to GenSpace or BioCurious).
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Required || Adobe CQ Admin || Charlotte, NC || 6+ Months || Phone And Skype
Dear Partner
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Adobe CQ Admin
Charlotte, NC
6+ Months
Phone And Skype
• 5+ years' experience as Systems Admin
• Experience administrating Adobe CQ
• WebSphere experience
• Red hat enterprise Linux experience
• OS, Linux or DB2
o Must have web infrastructure
o Ecommerce experience
They will be working on the infrastructure for Lowes.com, digital, and mobility.
• 5+ years working in a AIX/Linux environment (AIX is IBM's version of Linux)
• 5+ years' experience working with VMware
• 5+ years working with WebSphere
Thanks and Regards,
Arpit Arora | Sr.Technical Recruiter
Technology Resource Group Inc.
3736 Hillsdale Court Santa Clara, CA 95051
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Required || Business Object/ BODeveloper || NYC,NY || 6+ Months|| GC/Citizen || Phone And F2F
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SQL Server Developer / SAP Business Objects BI Developer
NYC,NY
6+ Months
Phone And F2F
Candidates must be local and available for a F2F interview.
SQL Server Development skills – 70%.
BI Skills – 30%.
Must be a strong SQL Server Developer with strong experience with Business Objects Products.
Purpose of Position:
To design, develop, enhance and maintain reports in SAP BusinessObjects BI Platform and SQL Server database environment.
Key Responsibilities:
- Interact with end users and business analysts to understand requirement.
- Database object development in SQL Server environment
- Report development in various SAP BusinessObjets BI Platform components, including Crystal Report, Universe, Web Intelligent and Dashboard
- Source Code version control in Team Foundation Server.
- Develop report documentation for deployment
Qualifications/Requirements:
· Three years of experience in a health care related environment;
· College degree in a related field;
· Excellent MS SQL Server skills
· Two years' experience with SAP BusinessObjects BI platform development
· Knowledge of Visual Studio .Net Development
· Ability to adapt to different projects and assignments;
· Team player with strong analytical skills
Thanks and Regards,
Arpit Arora | Sr.Technical Recruiter
Technology Resource Group Inc.
3736 Hillsdale Court Santa Clara, CA 95051
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Urgent Client Need: InstallShield Consultant; Bedford, Mass; 7+ Months Contract
Hi,
This is Niranjan from Vision3solutions; hope you are doing well.
Please go through below description and reply with your resume, contact details and current location, if you feel comfortable
Title: InstallShield Consultant
Location: Bedford, Mass
Duration: 7+ Months Contract
Locals Only
Job Description
Project
Build the next generation enterprise Installer platform for TruCare from the ground up to automate the process of installing and configuring Tomcat 7 and Java EE Web applications in multi-server, clustered topologies. Automate the deployment and configuration of Java EE Web applications (WARs) on Tomcat servers, and the installation and configuration SQL Server databases for these applications. Additionally, build the necessary automation for upgrading JBoss 5.1.0 instances to Tomcat 7 in existing older-version installations of TruCare, migrating Java EE configurations from JBoss 5.1.0 to Tomcat 7, deploying updated Java EE Web applications (WARs) on the upgraded Tomcat instances, and executing the data migration and schema upgrade SQL scripts on SQL Server databases.
As part of a small, focused Scrum team, the InstallShield consultant will collaborate with the product manager and other build and install engineers to convert requirements and functional specifications into detailed technical designs, and implement those designs into a functional Installer solution.
· Requirements
· Advanced expertise with FlexeraInstallShield Professional or Premier (2012 or later).
· 2+ years' experience building enterprise-class Installer solutions for installing, deploying and configuring server-side applications and databases (Java or .NET) using InstallShield
· Knowledge and experience with Subversion, Maven, Ant and Atlassian Bamboo.
· Familiarity with the Java EE framework and Tomcat is desirable.
· SQL knowledge preferred.
Thanks& Regards
Niranjan Kumar
Vision3 Solutions Inc
8155 Cavendish Place,Suwanee, GA 30024
Contact No: (678) 554-4785
Email: niranjan.kumar@vision3solutions.com
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Urgent Client Need: InstallShield Consultant; Bedford, Mass; 7+ Months Contract
Please send your resumes at niranjan.kumar@vision3solutions.com
Hi,
This is Niranjan from Vision3solutions; hope you are doing well.
Please go through below description and reply with your resume, contact details and current location, if you feel comfortable
Title: InstallShield Consultant
Location: Bedford, Mass
Duration: 7+ Months Contract
Locals Only
Job Description
Project
Build the next generation enterprise Installer platform for TruCare from the ground up to automate the process of installing and configuring Tomcat 7 and Java EE Web applications in multi-server, clustered topologies. Automate the deployment and configuration of Java EE Web applications (WARs) on Tomcat servers, and the installation and configuration SQL Server databases for these applications. Additionally, build the necessary automation for upgrading JBoss 5.1.0 instances to Tomcat 7 in existing older-version installations of TruCare, migrating Java EE configurations from JBoss 5.1.0 to Tomcat 7, deploying updated Java EE Web applications (WARs) on the upgraded Tomcat instances, and executing the data migration and schema upgrade SQL scripts on SQL Server databases.
As part of a small, focused Scrum team, the InstallShield consultant will collaborate with the product manager and other build and install engineers to convert requirements and functional specifications into detailed technical designs, and implement those designs into a functional Installer solution.
· Requirements
· Advanced expertise with FlexeraInstallShield Professional or Premier (2012 or later).
· 2+ years' experience building enterprise-class Installer solutions for installing, deploying and configuring server-side applications and databases (Java or .NET) using InstallShield
· Knowledge and experience with Subversion, Maven, Ant and Atlassian Bamboo.
· Familiarity with the Java EE framework and Tomcat is desirable.
· SQL knowledge preferred.
Thanks& Regards
Niranjan Kumar
Vision3 Solutions Inc
8155 Cavendish Place,Suwanee, GA 30024
Contact No: (678) 554-4785
Email: niranjan.kumar@vision3solutions.com
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Re: [DIYbio] Re: Anyone in the Sacramento/Davis area interested in making a bio hackerspace?
Yes. Counter Culture Labs is only 20 minutes away from me. So much closer.
Marnia: if the trip to Oakland would be more manageable, Counter Culture Labs might be an option for you?
On Dec 23, 2011 12:30 PM, "Marnia Johnston" <marnia@gmail.com> wrote:--yeah, just as bad as the trek to Palo Alto or Sunnyvale.On Thu, Dec 22, 2011 at 10:34 PM, CodonAUG <elsbernd@gmail.com> wrote:
I think you would hate that commute.
On Dec 22, 12:23 pm, Marnia Johnston <mar...@gmail.com> wrote:
> I'm in Concord but that might still be pretty far away.
>
>
>
>
>
>
>
> On Wed, Dec 21, 2011 at 10:49 PM, CodonAUG <elsbe...@gmail.com> wrote:
> > Is there anyone in the Sacramento/Davis area interested in making a
> > bio hackerspace (something akin to GenSpace or BioCurious).
>
> > --
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