No. No one of Salmonella species has possibility to use lactose, so they don't have any parts of lactose operon. LacI should be inserted simultaneously with LacY and LacZ. Also, I've analysed Salmonella 16s rRNA sequencies on NCBI and there are differencies in RBS of E. coli and Salmonella(but yes, they are VERY similar). But, when I'll obtain "building blocks" with E.coli promoters and RBS I'll try to use them as well. And if I'll obtain not very good results - I'll try to use this.
Am Samstag, 30. Mai 2015 17:02:00 UTC+3 schrieb Mega [Andreas Stuermer]:
-- Am Samstag, 30. Mai 2015 17:02:00 UTC+3 schrieb Mega [Andreas Stuermer]:
E coli and Salmonella are more or less related. So I guess the coli promoter woud likely work without modification. Does Salmonella have a lac repressor?
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