Interesting info. Thanks a lot! Are known functioning modifications of LacI, LacZ, LacY? What if prducts of these genes would be LVA marked for faster degradation?
Am Samstag, 30. Mai 2015 17:42:34 UTC+3 schrieb Michael Tellier:
-- Am Samstag, 30. Mai 2015 17:42:34 UTC+3 schrieb Michael Tellier:
Interestingly, most Salmonella genomes have lost the lac operon, which seems to hinder its pathogenicity by down-regulating the genes involved in the flagelles. So if you put your construct in Salmonella, your gene will be always expressed since LacI is likely to be absent. You might need to add the LacI gene on your construct to express the repressor.About Salmonella and the absence of the lac operon:http://link.springer.com/article/10.1007%2Fs00284-014- 0720-7
On Saturday, 30 May 2015 15:02:00 UTC+1, Mega [Andreas Stuermer] wrote:E coli and Salmonella are more or less related. So I guess the coli promoter woud likely work without modification. Does Salmonella have a lac repressor?
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