Sure, I dont have it with me right know, but it was something like this:
I inoculated colonies in LB and let them grow overnight. In the morning I diluted it in M9 medium and let it grow for 4 hours, then I did the first measurement (T0), I measured the OD and fluorescence. After another 1 hour of incubation I measured the OD and fluorescence again (T1).
At T0 the fluorescence was around 50 and at T1 it was around 70, the OD I cant remember. It would be great to get an estimate of the GFP concentration inside the cell.
Thanks!
Hi guys!I would like to rank in strength a few promoters I have. The promoters are cloned upstream GFP, so I used a spectrophotometer (485 nm excitation 525 nm emission) to measure the GFP in E. coli, just like the paper "Measuring the activity of BioBrick promoters using an in vivo reference standard". I did everything, and now I have a some numbers, but I don't know how to turn it in something useful. Anyone know which formula should I use to get a estimate of the GFP or mRNA concentration inside the cell?Thanks a lot!
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